Advancements in Palynology: Enhancing Plant Breeding through Pollen Studies

Palynology, the study of pollen and spores, has emerged as a vital discipline in plant science, with applications extending into plant breeding. This review explores the role of palynology in understanding reproductive biology, enhancing breeding programs, overcoming hybridization barriers and addressing challenges like male sterility, stress tolerance and species compatibility. Advanced techniques like transcriptomics reveal gene expression profiles, while proteomics and metabolomics provide insights into fertility and stress responses. Pollen-mediated transformation and CRISPR-Cas9 gene editing facilitate targeted trait improvements, with pollen-specific promoters aiding hybrid breeding. Additionally, molecular tools support pollen viability testing, gene flow studies, and stress tolerance research, contributing to hybrid seed production and environmental adaptability. Palynology also ensures biosafety by tracking transgenic pollen, making it an integral component of sustainable crop improvement strategies.

Case studies illustrate its impact on cereals, legumes and horticultural crops. The article concludes by highlighting challenges and future directions for integrating palynological studies with emerging technologies to improve plant breeding outcomes. Plant breeding aims to develop superior crop varieties with desirable traits such as higher yields, resistance to biotic and abiotic stresses, improved quality and adaptability to diverse environments. Successful breeding hinges on understanding the reproductive biology of plants, particularly pollen, which serves as the carrier of male gametes