A Novel Study of Shigella Biosurfactants Like Molecule Produced and Assessed Anaerobically

Shigella has settled on extremely sophisticated mechanisms to disrupt host cell progressions to endorse infection, escape immune exposure, and avoid bacterial clearance. The design of new therapeutic targets for the optimal management of Shigella infection must give a follow-up action target in the live anaerobic cycle. The aim of this present work is to explore the ability of Shigella to produce and secrete biosurfactant like molecules anaerobically. This study describes the isolation of 93 Shigella spp. candidates, from hospital and environmental samples. These isolates, plus lab strains, were tested in a comprehensive in vitro screening regime including biochemical and microbiological analysis, emulsification index (EI24), the DNA technology and sequencing, swarming test, the biofilm formation and S. flexneri were able to secrete and produce biosurfactant with the percentage ranging from 68 to 100%. Strains were also swarming in semisolid media, with swarm diameters ranging from 75 to 85 mm on soft agar (0.5%) in the absence of oxygen. This study showed that Shigella were unable to invade eggs in the absence of oxygen, with zero (0%) invasion rates, but retained their ability to form biofilms. The invasion rate was zero in anaerobiosis and more than 50% in aerobiosis. These results show that biosurfactants are produced in cotranslational pathways and are secreted into the extracellular medium through the Shigella type three secretion system (T3SS). Shigella biosurfactants are involved in biofilm formation and secrete into the extracellular medium via the phenomenon known as the "T3SS leakage."